Journal: Experimental and Therapeutic Medicine

Article Title: A mix of ginger phenols exhibits anti‑adipogenic and lipolytic effects in mature adipocytes derived from 3T3‑L1 cells

doi: 10.3892/etm.2023.12035

Figure Lengend Snippet: Cell viability dose-response curve of phenol-mix. (A) 3T3-L1 cells were treated with the phenol-mix at 1, 2, 3, 4 and 5 µg/ml during adipogenic differentiation and cell viability was determined using MTT assay. (B) Mature 3T3-L1 adipocytes were treated with the phenol-mix at 1,2,3,4 and 5 µg/ml for 48 h and cell viability was measured using MTT assay. Each bar represents the mean ± standard deviation of three experiments. ** P<0.01 and *** P<0.001 vs. positive control. NC, negative control (3T3-L1 cells); PC, positive control (mature 3T3-L1 adipocytes); phenols-pre, 3T3-L1 cells stimulated with the phenols mix during adipogenic differentiation; phenols-post, mature 3T3-L1 adipocytes stimulated with the phenols mix.

Article Snippet: On day 3, the medium was replaced with DMEM/F-12 supplemented (cat. no. 21041025; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (cat. no. 16000044; Thermo Fisher Scientific, Inc.), 1% antibiotics and an adipogenic cocktail including 500 µM IBMX, 1 µM dexamethasone, 1.5 µg/ml insulin and 1 µM rosiglitazone (3T3-L1 Differentiation Kit; cat. no. SIG-DIF001-1KT; Merck KGaA) for 3 days.

Techniques: MTT Assay, Standard Deviation, Positive Control, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: A mix of ginger phenols exhibits anti‑adipogenic and lipolytic effects in mature adipocytes derived from 3T3‑L1 cells

doi: 10.3892/etm.2023.12035

Figure Lengend Snippet: Dose-response curve of the main ginger phenols mix effects on lipid content. (A) 3T3-L1 cells were treated with the mix of the main ginger phenols at various concentrations (1,2,3,4 and 5 µg/ml) during adipogenic differentiation and lipid content was measured. (B) Mature 3T3-L1 adipocytes were treated with the mix of the main ginger phenols at various concentrations (1,2,3,4 and 5 µg/ml) for 48 h and lipid content was measured. Each bar represents the mean ± standard deviation of three experiments. ** P<0.01 and *** P<0.001 vs. positive control. NC, negative control (3T3-L1 cells); PC, positive control (mature 3T3-L1 adipocytes); phenols-pre, 3T3-L1 cells stimulated with the phenols mix during adipogenic differentiation; phenols-post, mature 3T3-L1 adipocytes stimulated with the phenols mix.

Article Snippet: On day 3, the medium was replaced with DMEM/F-12 supplemented (cat. no. 21041025; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (cat. no. 16000044; Thermo Fisher Scientific, Inc.), 1% antibiotics and an adipogenic cocktail including 500 µM IBMX, 1 µM dexamethasone, 1.5 µg/ml insulin and 1 µM rosiglitazone (3T3-L1 Differentiation Kit; cat. no. SIG-DIF001-1KT; Merck KGaA) for 3 days.

Techniques: Standard Deviation, Positive Control, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: A mix of ginger phenols exhibits anti‑adipogenic and lipolytic effects in mature adipocytes derived from 3T3‑L1 cells

doi: 10.3892/etm.2023.12035

Figure Lengend Snippet: Effects of phenol-mix on cell viability and lipid content. (A) The study groups were treated with 2 µg/ml phenol-mix and (A) Cell viability (MTT assay) and (B) Lipid content were measured. (C) Representative images of the lipid content in the four groups after Oil Red O staining (magnification, x40). Each bar represents the mean ± standard deviation of three experiments. *** P<0.001 vs. positive control. NC, negative control (3T3-L1 cells); PC, positive control (mature 3T3-L1 adipocytes); phenols-pre, 3T3-L1 cells stimulated with the phenols mix during adipogenic differentiation; phenols-post, mature 3T3-L1 adipocytes stimulated with the phenols mix.

Article Snippet: On day 3, the medium was replaced with DMEM/F-12 supplemented (cat. no. 21041025; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (cat. no. 16000044; Thermo Fisher Scientific, Inc.), 1% antibiotics and an adipogenic cocktail including 500 µM IBMX, 1 µM dexamethasone, 1.5 µg/ml insulin and 1 µM rosiglitazone (3T3-L1 Differentiation Kit; cat. no. SIG-DIF001-1KT; Merck KGaA) for 3 days.

Techniques: MTT Assay, Staining, Standard Deviation, Positive Control, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: A mix of ginger phenols exhibits anti‑adipogenic and lipolytic effects in mature adipocytes derived from 3T3‑L1 cells

doi: 10.3892/etm.2023.12035

Figure Lengend Snippet: Effects of the main ginger phenols mix on glycerol-release activity. 3T3-L1 cells were treated with 2 µg/ml of the mix of the main ginger phenols during adipogenic differentiation, while mature 3T3-L1 adipocytes were treated with the same dose for 48 h. Glycerol determination in supernatant was performed using VITROS 350 Chemistry System following the manufacturer's instructions. Each bar represents the mean ± standard deviation of three experiments. *** P<0.001 vs. positive control. NC, negative control (3T3-L1 cells); PC, positive control (mature 3T3-L1 adipocytes); phenols-pre, 3T3-L1 cells stimulated with the phenols mix during adipogenic differentiation; phenols-post, mature 3T3-L1 adipocytes stimulated with the phenols mix.

Article Snippet: On day 3, the medium was replaced with DMEM/F-12 supplemented (cat. no. 21041025; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (cat. no. 16000044; Thermo Fisher Scientific, Inc.), 1% antibiotics and an adipogenic cocktail including 500 µM IBMX, 1 µM dexamethasone, 1.5 µg/ml insulin and 1 µM rosiglitazone (3T3-L1 Differentiation Kit; cat. no. SIG-DIF001-1KT; Merck KGaA) for 3 days.

Techniques: Activity Assay, Standard Deviation, Positive Control, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: A mix of ginger phenols exhibits anti‑adipogenic and lipolytic effects in mature adipocytes derived from 3T3‑L1 cells

doi: 10.3892/etm.2023.12035

Figure Lengend Snippet: Effects of the main ginger phenols mix on pro-adipogenic and -lipogenic genes expression. (A) CCAAT enhancer-binding protein α, (B) peroxisome proliferator-activated receptor-γ, (C) fatty acid binding protein 4, (D) acetyl-coenzyme A carboxylase and (E) fatty acid synthase mRNA levels in 3T3-L1 mature adipocytes were examined using reverse transcription-quantitative PCR. Each bar represents the mean ± standard deviation of three experiments. * P<0.05 and *** P<0.001 vs positive control. ACACA, acetyl-coenzyme A carboxylase; C/EBPα, CCAAT enhancer-binding protein α; FABP4, fatty acid binding protein 4; FASN, fatty acid synthase; PPAR-γ, peroxisome proliferator-activated receptor-γ; NC, negative control (3T3-L1 cells); PC, positive control (mature 3T3-L1 adipocytes); phenols-pre, 3T3-L1 cells stimulated with the phenols mix during adipogenic differentiation; phenols-post, mature 3T3-L1 adipocytes stimulated with the phenols mix.

Article Snippet: On day 3, the medium was replaced with DMEM/F-12 supplemented (cat. no. 21041025; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (cat. no. 16000044; Thermo Fisher Scientific, Inc.), 1% antibiotics and an adipogenic cocktail including 500 µM IBMX, 1 µM dexamethasone, 1.5 µg/ml insulin and 1 µM rosiglitazone (3T3-L1 Differentiation Kit; cat. no. SIG-DIF001-1KT; Merck KGaA) for 3 days.

Techniques: Expressing, Binding Assay, Real-time Polymerase Chain Reaction, Standard Deviation, Positive Control, Negative Control